Model for studying Clostridium botulinum neurotoxin using differentiated motor neuron-like NG108-15 cells

–Receptor Antagonist, on Differentiated NG108-15 Cells

We have reported previously that the concentration of intracellular Ca 2+ evoked by serotonin (5-HT) was significantly augmented in differentiated NG108-15 (NG) cells treated with dibutyryl cAMP and the enhanced response occurred via 5-HT 3 receptors. We investigated changes in the characteristics for specific binding of [ 3 H]LY-278584 (a specific antagonist of the 5-HT 3 receptor) on membrane...

Nucleotide sequence of Clostridium botulinum C1 neurotoxin.

Daniel Hauser, Melvin W.Eklund, Hisao Kurazono, Thomas Binz, Heiner Niemann, D.Michael Gill, Patrice Boquet and Michel R.Popoff* Unite des Anaerobies and 1 Unite des Antigenes Bacteriens, Institut Pasteur, 28 rue du Dr. Roux-75724 Paris cedex 15, France, Northwest and Alaska Fisheries Center, 2725 Montlake Boulevard East, Seattle, WA 98112, USA, lnstitut fur Mikrobiologie an der Bundesforschung...

PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells

Objective(s): Cell therapy has provided clinical applications to the treatment of motor neuron diseases. The current obstacle in stem cell therapy is to direct differentiation of stem cells into neurons in the neurodegenerative disorders. Biomaterial scaffolds can improve cell differentiation and are widely used in translational medicine and tissue engineering. The aim...

Purification of Clostridium botulinum type A neurotoxin.

Conjugative Botulinum Neurotoxin-Encoding Plasmids in Clostridium botulinum

BACKGROUND Clostridium botulinum produces seven distinct serotypes of botulinum neurotoxins (BoNTs). The genes encoding different subtype neurotoxins of serotypes A, B, F and several dual neurotoxin-producing strains have been shown to reside on plasmids, suggesting that intra- and interspecies transfer of BoNT-encoding plasmids may occur. The objective of the present study was to determine whe...